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Evidence Guide: FBPFST4004 - Perform microbiological procedures in the food industry

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

FBPFST4004 - Perform microbiological procedures in the food industry

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare for safe microbiological work using aseptic techniques

  1. Select work area, equipment and instructions for the safe handling of materials that may contain microorganisms
  2. Wear personal protective apparel
  3. Select relevant emergency equipment for timely response to microbiological accidents
  4. Apply correct disinfection procedures to work areas before and after use
  5. Apply standard precautions when handling biological materials
Select work area, equipment and instructions for the safe handling of materials that may contain microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Wear personal protective apparel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select relevant emergency equipment for timely response to microbiological accidents

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply correct disinfection procedures to work areas before and after use

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply standard precautions when handling biological materials

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Process microbiological samples and undertake microscopy

  1. Prepare thin smears of samples and stain
  2. Prepare liquid films of specimens for direct observation
  3. Concentrate relevant samples to facilitate microscopy
  4. Set up stereo and compound microscopes and identify causes of variations in image quality
  5. Examine dry, wet and stained microbiological specimens
  6. Clean and store microscopes
Prepare thin smears of samples and stain

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare liquid films of specimens for direct observation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Concentrate relevant samples to facilitate microscopy

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Set up stereo and compound microscopes and identify causes of variations in image quality

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Examine dry, wet and stained microbiological specimens

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean and store microscopes

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply aseptic techniques correctly to cultivate and isolate microorganisms

  1. Prepare media for culturing cells
  2. Perform aseptic transfers of microorganisms to prepared liquid and solid media
  3. Streak bacteria onto media plates to isolate single colonies using aseptic technique
  4. Select temperature conditions and gaseous environments suitable for the growth of a range of common microorganisms
Prepare media for culturing cells

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform aseptic transfers of microorganisms to prepared liquid and solid media

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Streak bacteria onto media plates to isolate single colonies using aseptic technique

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select temperature conditions and gaseous environments suitable for the growth of a range of common microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Estimate the number of microorganisms in food and water samples

  1. Prepare samples for testing
  2. Carry out serial dilutions aseptically
  3. Estimate and record bacterial growth in the sample
  4. Calculate the bacterial load of the sample and report the results
Prepare samples for testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Carry out serial dilutions aseptically

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Estimate and record bacterial growth in the sample

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Calculate the bacterial load of the sample and report the results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform and interpret tests to assist in the identification of common bacterial genera

  1. Perform tests on pure cultures to assist in the identification of major bacterial groups
  2. Prepare pure cultures selected from common bacterial genera
  3. Select and prepare stained specimens to demonstrate features and cellular characteristics of major bacterial groups
Perform tests on pure cultures to assist in the identification of major bacterial groups

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare pure cultures selected from common bacterial genera

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select and prepare stained specimens to demonstrate features and cellular characteristics of major bacterial groups

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply quality assurance procedures commonly used in a food testing laboratory

  1. Apply controls used to monitor accuracy and precision of results in a microbiological laboratory
  2. Perform all tests in accordance with enterprise quality procedures
  3. Record and report all test data appropriately
Apply controls used to monitor accuracy and precision of results in a microbiological laboratory

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform all tests in accordance with enterprise quality procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record and report all test data appropriately

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Interpret the results of laboratory testing and relate to the production plan

  1. Access laboratory test results
  2. Analyse laboratory tests to identify allowable variances and critical limits in production
  3. Make adjustments to recipes or operating procedures to ensure critical limits are complied with
  4. Establish the need for further certified testing
Access laboratory test results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Analyse laboratory tests to identify allowable variances and critical limits in production

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Make adjustments to recipes or operating procedures to ensure critical limits are complied with

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Establish the need for further certified testing

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge